Fig. 3

Ref1 reduction lowers G4C2 RNA and dipeptide abundance. a Ref1 RNAi suppresses G4C2 repeat-mediated neurodegeneration. Genotypes. Control: UAS-(G4C2)49, YH3-GAL4/UAS-control RNAi (JF01355). Ref1: UAS-(G4C2)49, YH3-GAL4/UAS-Ref1 RNAi (HMS01301). Quantification of retinal area shown on the right. ***p < 0.001, two-tailed Student’s t-test. b Ref1 knockdown reduces G4C2 mRNA levels in Drosophila heads. d Ref1 knockdown reduces GR-GFP protein levels in the Drosophila eye. e Quantification of GR-GFP signal. Genotypes for (b) and (c). Control RNAi: (G4C2)44 [GR-GFP], YH3/ UAS-control RNAi (JF01355). Ref1 RNAi: (G4C2)44 [GR-GFP], YH3/ UAS-Ref1 RNAi (HMS01301). ***p < 0.001 two-tailed Student’s t-test. For all graphs, individual data points are shown with mean +/− standard deviation