Fig. 2

Tau isoform-oriented mapping into four repeat only (4R+/3R-) regions for PSP type and three and four repeat (3R+/4R+) regions for AD type distribution. Representative immunostaining for four-repeat (4R) tau (RD4, green) and three-repeat (3R) tau (RD3, red) in these twelve regions. The left set columns demonstrated 4R specific immunoreactivity (IR) without 3R IR in GPI (a, g), STN (b, h), RN (c, i), PN (d, j), ION (e, k) and DN (f, l), which replicates PSP-type distribution with glial involvement (arrows). In contrast, the right set columns demonstrated both 4R and 3R tau IR in PC (m, s), HF (n, t), CGM (o, u), SN (p, v), LC (q, w) and RPN (r, x), which replicates AD-type distribution. Glial lesions are exclusively positive for RD4 (arrows) but negative for RD3. Bars: 100 μm