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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: AQP4ex is crucial for the anchoring of AQP4 at the astrocyte end-feet and for neuromyelitis optica antibody binding

Fig. 4

AQP4 and AQP4ex supramolecular organization in mouse brain. a BN-PAGE analysis of AQP4 and AQP4ex expression and supramolecular aggregation in the cerebrum and cerebellum of wild-type (WT), heterozygous (HET) and AQP4ex-KO (Ex-KO) mice. The high molecular weight of AQP4 pools (OAP) and the tetrameric form of AQP4 (T) are indicated. b Four different pools of AQP4 were analyzed for the band shift analysis. The profile of the cerebrum AQP4 pools (top) and the cerebellum AQP4 pools (bottom) for the three animals is shown and the red dotted lines represent the shift of the analyzed pools for the cerebrum (2–5) and the cerebellum (3–6). c AQP4 isoform interaction by IP experiment analysis. Brain homogenates were immunoprecipitated with anti-AQP4ex (IP AQP4ex) and anti-AQP4 (IP AQP4) antibody and subjected to immunoblot analysis with anti-AQP4 (left) and anti-AQP4ex (middle) antibodies, respectively. Samples immunoprecipitated with anti-AQP4 antibody were probed for AQP4 as control (right). The top band at 35 kDa corresponds to AQP4ex, and the bottom bands at 30 and 32 kDa correspond to the canonical AQP4 isoforms. The first two lanes of the immunoblot of the left panel contain brain lysates probed with AQP4 antibodies loaded as positive controls

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