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Table 1 MSA prion infection of four different Tg mouse astrocyte cultures expressing human α-synuclein

From: Replication of multiple system atrophy prions in primary astrocyte cultures from transgenic mice expressing human α-synuclein

Astrocyte donor mice Human α-synuclein levels relative to mousea Mouse α-synuclein Relative rank of human α-synuclein levelsb pSyn (S129) intensity after exposure to MSA patient–derived or [TgM83-passaged] brain homogenatec
0 dpe 7 dpe 14 dpe 21 dpe
Tg(SNCA*A53T)M83+/+ 4.6x +/- 0.8* Yes ++++ 1[0.5] 5.7[2.9] 14.2 [8.9] 23[13.8]
Tg(SNCA*A53T)M83+/– 3.3x +/- 0.5* Yes +++ 1[0.5] 2[0.6] 6.7[3.3] 10.7[6.3]
Tg(SNCA+/+)Nbm 1.3 – 2x§ No +++ 1.1[0.7] 3.4[1.5] 11[10.5] 18.7[22.0]
Tg(SNCA*A53T)Nbm 1.3 – 2x§ No ++ 1[0.6] 2.2[0.6] 9.6[2.3] 12.9[3.5]
Tg(SNCA*A30P)Nbm 1.3 – 2x§ No +++ 0.9[0.5] 1.4[0.6] 3.7[2.9] 6.7[3.5]
Snca 0/0 None ¥ No - 0[0] 0[0] 0[0] 0[0]
  1. aLevels of human α-synuclein in brain cortex relative to levels of endogenous mouse α-synuclein—* [28]; § [41]; ¥ [10].
  2. bmAb Syn211 was used to estimate human α-synuclein levels in cultured Tg astrocytes (20 μg of total protein analyzed by Western blot).
  3. cmAb pSyn (S129) staining intensity in MSA-infected Tg astrocytes. Cultures were exposed to either 0.5% MSA35 patient–derived or 0.5% mouse-passaged MSA [(MSA2→TgM83) →TgM83] brain homogenate (values in brackets). Data represent the fluorescent signal intensity of phosphorylated α-synuclein (S129) immunostaining per cell (x104) summarized from experiments described in Figs. 1, 2, 6, and S8. Details of data acquisition and analysis are described in the Material and Methods. Note: The initial amounts of MSA prions in the two different inocula were not normalized