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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Replication of multiple system atrophy prions in primary astrocyte cultures from transgenic mice expressing human α-synuclein

Fig. 7

Astrocyte cultures infected with MSA prions do not show retraction of neuronal dendritic spines. TgM83+/− astrocytes were infected with MSA prions and maintained in an FBS-free medium for 21 days. Freshly isolated neuronal cultures from either (a, c) TgM83+/− or (b, d) Tg(SNCA+/+)Nbm P0 mice were plated on a layer of (a, b) control or (c, d) MSA-infected astrocytes. Cells were then cultured in FBS-free medium for 14 days. Immunostaining was carried out with pSyn (S129) (red) to reveal MSA prion infection and IIIβ-tubulin (white) to assess neuronal integrity. Insets of dashed areas are shown on right. Nuclei were stained with DAPI (blue). Scale bars, 50 μm

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