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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Replication of multiple system atrophy prions in primary astrocyte cultures from transgenic mice expressing human α-synuclein

Fig. 1

α-Synuclein accumulation and phosphorylation at serine 129 in primary astrocytes exposed to human MSA brain homogenate. a Representative images of immunohistochemical detection of α-synuclein deposits in a patient sample, MSA35. Brain tissue from pons (left) and occipital cortex (right). Tissues were immunostained for phosphorylated α-synuclein at serine 129 [pSyn (S129), brown], and nuclei are in blue. Scale bars, 100 μm. b Quantification of pSyn (S129) signal intensity in TgM83+/+ astrocytes exposed to MSA35 brain tissue from pons (dark blue circles), occipital cortex (white matter; pink circles), and occipital cortex (grey matter; grey circles). Only tissue from the midbrain (pons) region induced rapid accumulation of pSyn (S129) over time. The signal intensity was normalized by cell count. Data are plotted with mean (n = 3) and analyzed using an unpaired t-test. c Representative immunographs of primary TgM83+/+ astrocytes exposed to 0.5% MSA35 brain homogenate (pons) for 48 h and further cultured in fresh media up to 21 days post-exposure (dpe). Cells were immunostained for total human α-synuclein (αSyn, green), pSyn (S129) (red), and glial fibrillary acidic protein (GFAP, white). Merge of all three channels is shown in the bottom row. Nuclei were stained with DAPI (blue). Scale bars, 50 μm

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