Fig. 2

Histopathological changes in 79A scrapie-infected retinas. a TUNEL staining of mock-infected mouse shows no evidence of apoptosis. b Scrapie-infected tgGFP/RFP mouse retina with TUNEL-positive nuclei (arrow) in the ONL indicating active apoptosis at 124 dpi. c Scrapie-infected C57BL/10 retina showing TUNEL-positive nuclei in the ONL (arrow) at 144 dpi. d Anti-GFAP staining of mock-infected retina is restricted to perivascular astrocytes (arrow) in the GC layer. e & f In tgGFP/RFP and C57BL/10 scrapie-infected retinas, GFAP-positive (red) processes of activated Müller cells (astrocytes) extend from GC layer to outer limiting membrane (arrow) of the ONL. g Anti-Iba1 staining of mock-infected retina shows a few Iba1-positive microglia (arrows) in the IPL and OPL. h & i In both tgGFP/RFP and C57BL/10 scrapie-infected retinas, Iba1-positive microglia are now also in ONL, OS and IS regions (arrows). These cells have enlarged cell bodies and thick processes typical of activated microglia. Scale bar = 50 μm, each column contains images from one representative mouse