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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Targeting the β2-integrin LFA-1, reduces adverse neuroimmune actions in neuropathic susceptibility caused by prenatal alcohol exposure

Fig. 3

I.t. BIRT-377 injection significantly decreases IL-1β expression. a-b Separate analysis of IL-1β and IL-10 immunoreactivity within the dorsal horn spinal cord at Day 4 post-injection (Day 32 after minor CCI; rats from data shown in Fig. 1) reveal that IL-1β is significantly decreased compared to vehicle injected rats with minor CCI regardless of prenatal exposure. b Significant differences in IL-10 expression are observed in between Sac-sham rats and Sac-minor CCI rats given vehicle, whereas no differences are observed between all experimental conditions in PAE rats. Sac rats with minor CCI given vehicle reveal elevated IL-10 immunoreactivity compared to allodynic PAE rats given vehicle. PAE rats lack the compensatory IL-10 elevation to minor injury. Representative images of (c) IL-1β and (d) IL-10 immunoreactivity used in IHC analysis are shown for Sac/Sham/Veh, Sac/1-Sut/Veh, Sac/1-Sut/BIRT, PAE/Sham/Veh, PAE/1-Sut/Veh, and PAE/1-Sut/BIRT at either 20x for IL-1β and 40x for IL-10. The white dashed line represents a portion of the superficial dorsal horn as is the ROI under examination. “1-sut CCI” = “minor CCI”. N = 4–5 rats per group. Asterisks indicate p < 0.05

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