Fig. 8

TRPM3/MIR204 promoter methylation analysis and upregulation of miR-204 expression upon treatment with HDAC inhibitors in medulloblastoma cells. a. Genome wide methylation data of the MAGIC cohort of 763 medulloblastomas was analyzed for methylation at the CpG island at the TRPM3 / MIR204 promoter. The scatter plot shows the Beta values for probe cg23553442 located in the CpG island in the four molecular subgroups. b. Y axis denotes fold change in the expression levels of miR-204 after treatment of the indicated medulloblastoma cell line with HDAC inhibitors Sodium valproate (VPA) and Trichostatin A (TSA). c. Western blot analysis showing H3K9 acetylation levels in the indicated medulloblastoma cell line in the vehicle control (VC) and the HDAC inhibitor-treated cells. The numbers below the western blots indicate the fold change in the indicated protein level upon miR-204 expression using GAPDH as a house-keeping control. **, ***, ns indicate p ≤ 0.001, p ≤ .0001 and non-significant respectively