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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Pathogenic alpha-synuclein aggregates preferentially bind to mitochondria and affect cellular respiration

Fig. 4

Aggregated αSyn preferentially binds to mitochondria. a Mitochondria isolated from αSyn monomer-treated or PFF-treated neurons were separated by iodixanol gradient, and the presence of mitochondrial protein ATPIF1, total αSyn, and ps-αSyn was visualized by immunoblot analyses as indicated. The arrowhead indicates the position of full-length αSyn; the arrow indicates the position of truncated αSyn. b Isolated mitochondria with or without in vitro incubation with αSyn monomer or PFF for 30 min at room temperature were subjected to iodixanol gradient separation. PFF alone was also subjected to gradient separation as a control. The presence of the mitochondrial ATPIF1 or total αSyn in each fraction was visualized by immunoblot analyses as indicated

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