Fig. 5

Characterization of the immune environment of the nervous tissue of NMDAR-E-associated ovarian teratomas. Representative nervous tissue of NMDAR-E teratoma infiltrated by immune cells and stained by hematoxylin-phloxine-saffron (a, f, k) or immunolabeled with the neuronal marker NF (b, g, l), the CD3 T-cell marker (c, h, m), the CD20 B-cell marker (d, i, n), or the mature dendritic cell marker DC-LAMP (e, j, o). a-e Diffuse inflammatory infiltrate closely adjacent to neural tissue (a, dotted area) containing NF-stained neurons (b) and composed of T-cells (c) and B-cells (d), with some mature dendritic cells (e, see higher magnification in the upper right corner). (f-j) Tertiary Lymphoid Structure (TLS) next to a focus of neuroglial tissue (f, dotted area) containing some NF positive neurites (g), with segregated B- and T-cells (h, i) and mature dendritic cells (j, see higher magnification in the upper right corner). k-o Lymphoid infiltrates close to a foci of neuroglial tissue (k, dotted area) without NF expression (l) and composed of segregated T-cells (m) and B-cells (n). Note the absence of mature dendritic cells (o, see higher magnification in the upper right corner). Scale bars: 50 μm