Fig. 5
From: Tau exhibits unique seeding properties in globular glial tauopathy
Sarkosyl-insoluble GGT-tau promotes intracellular tau aggregation in primary mouse astrocytes. a Representative confocal images showing primary mouse astrocytes transduced with AAV-TauK317N and subsequently treated with sarkosyl-insoluble GGT-tau or AD-tau for comparison. E1 staining for human tau is in green and GFAP staining for astrocytes is in red. Nuclei were stained with Hoechst (scale bar = 10 μm). b Quantification of percentage of astrocytes that are transduced with AAV-TauK317N and display tau seeding-like puncta (*p < 0.05). c-d Changes in the level of tau aggregation in AAV-transduced astrocytes upon treatment with either sarkosyl-insoluble GGT-tau or AD-tau were detected by Western blot (c) and quantified based on band intensity (*p < 0.05; **p < 0.01) (d)