Fig. 4
From: Tau exhibits unique seeding properties in globular glial tauopathy
Sarkosyl-insoluble, fibril-like tau species implicated in GGT has the strongest seeding property. a A schematic diagram depicting the isolation method for different tau species from the brain samples. b Representative confocal images showing formation of the most seeding-induced puncta in the tau biosensor cell line upon incubation with sarkosyl-insoluble GGT-tau (scale bar = 100 μm). c The strongest tau seeding activity was detected in sarkosyl-insoluble GGT-tau as measured by the FRET flow cytometry assay. FRET density was normalized to the amount of total tau present in the sample. Data are presented as mean ± SD (***p < 0.001; ****p < 0.0001). d A representative electron microscopy (EM) image showing straight tau filaments in the sarkosyl-insoluble GGT-tau fraction