Fig. 3
From: Tau as a mediator of neurotoxicity associated to cerebral amyloid angiopathy
Tau hyperphosphorylation and misfolding in Tg-FDD mice. a WB analysis of brain lysate from WT and Tg-FDD 18 months old mice. WB using anti-BRI2 antibody was performed to confirm mice genotypes and Vinculin was used as a loading control. b Graph showing WB quantification of p-tau S396/S404/total tau ratio. ****p < 0.0001, Unpaired Student’s t test. c Graph showing WB quantification of p-tau T231/total tau ratio. ****p < 0.0001, Unpaired Student’s t test. d Graph showing WB quantification of MC1 tau/total tau ratio. ****p < 0.0001, Unpaired Student’s t test. For all three quantifications (b-d) error bars represented ± SD (n = 5). Values from WT mice were considered as 100%. (e) Endogenous murine tau mRNA levels measured by qRT-PCR did not change in Tg-FDD mice in comparison with WT mice (n = 5). f WB analysis of brain insoluble fractions from WT and Tg-FDD demonstrated the presence of ADan insoluble aggregates in Tg-FDD detected using the anti-ADan antibody (left blot). No tau (using tau-5 antibody) was detected in the insoluble fraction neither from WT nor Tg-FDD mice (right blot)