Fig. 1
From: Tau as a mediator of neurotoxicity associated to cerebral amyloid angiopathy
Expression of BRI2 bearing the Danish mutation promotes tau phosphorylation. a WB analysis of lysates from HEK tau-P301L cells transfected with WT BRI2 or mutant BRI2. b Graph showing WB quantification of p-tau S396/S404/total tau and p-tau T231/total tau ratio. For p-tau S396/S404 analysis; *p = 0.0372, Unpaired Student’s t test. For p-tau T231 analysis; *p = 0.0455, Unpaired Student’s t test. For both quantifications error bars represented ± SD (n = 3). Values of phospho-tau/total tau ratio in cells transfected with WT BRI2 were considered as 100%. c Orthogonal images of reconstructed three-dimensional views of p-tau T231 (green) and ADan (red) by confocal images of HEK cells expressing human tau-P301L transfected with mutant BRI2. Left panels shows staining with anti-p-tau T231 antibody (green), middle panels show staining with anti-ADan antibody (red), and right panels shows the merge of both signals, DAPI staining (blue) for nucleus plus cross-sectional images X-Z and Y-Z. Cross-sectional image in top panel, indicate ADan inclusions that colocalize with p-tau T231. In bottom panel, cross-sectional image indicates ADan inclusions that don’t colocalize with p-tau T231. Scale bar 15 μm