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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Inhibition of DYRK1A proteolysis modifies its kinase specificity and rescues Alzheimer phenotype in APP/PS1 mice

Fig. 4

L41 treatment promotes microglial cells recruitment leading to a decrease in aggregated amyloid load. a Laser confocal microscopy showing double staining, using 4G8 antibody (white) and anti-IBA1 (red), of hippocampal slices from vehicle- (n = 6, plaques = 79) or L41- (n = 6, plaques = 77) treated APP/PS1 mice. b Increased IBA1 immunoreactivity around amyloid plaque of L41-treated APP/PS1 mice, compared to vehicle-treated APP/PS1 mice, was observed (t-test, p < 0.05). c Western blot of hippocampus from vehicle (n = 6) or L41 (n = 7) treated APP/PS1 mice, showing levels of IBA1, CD68, IDE, and TREM2. d IBA1 protein levels were not statistically different in L41-and vehicle-treated APP/PS1 mice (t-test, ns). CD68, IDE, and TREM2 protein levels were increased in L41-treated compared to vehicle-treated APP/PS1 mice (CD68: t-test, p < 0.005; IDE: t-test, p < 0.05; TREM2: t-test, p < 0.005). e Laser confocal microscopy showing IBA1 positive cells with increased TREM2 protein and colocalization with Aβ species (4G8) in L41-treated compared to vehicle-treated APP/PS1 mice. f ELISA quantification of soluble Aβ40 and Aβ42 (MSD immunoassay) in vehicle- and L41-treated APP/PS1 mice hippocampus (n = 6/7 mice per group). There was no statistical differences between L41- and vehicle-treated APP/PS1 mice. g Laser confocal microscopy showing double staining with 4G8 antibody (white) and DAPI (blue), of hippocampal slices from vehicle- (n = 6) or L41- (n = 6) treated APP/PS1 mice. Area covered by the plaques was decreased in the hippocampus of L41-treated compared to vehicle-treated APP/PS1 mice (t-test, p < 0.05). Data represent the mean ± SEM and were analyzed by one-way ANOVA, followed by Tukey’s post hoc test or Student’s t-test. Significant differences between vehicle- and L41-treated APP/PS1 mice are indicated by #p < 0.05, ##p < 0.005, and ### p < 0.0005

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