Fig. 1

Identification of Leucettine L41 as a DYRK1A proteolysis inhibitor. a Control human hippocampus extract was incubated at 30 °C during 10 min with various concentrations of CaCl₂ (0 to 4,0 mM) or with 2 mM of EGTA. Proteins were then analyzed by western blot using the α-DYRK1A-Nter antibody. b Control human hippocampus extract was incubated with 0 mM of CaCl₂ or 2 mM of CaCl₂ and various pharmacological compounds including Harmine (har), Leucettine LeuI (LeuI) or Leucettine L41 (L41). Proteins were analyzed by western blot using the α-DYRK1A-Nter antibody. c Control mouse (C57Bl6) hippocampus extract was incubated at 30 °C during 10 min with various concentrations of CaCl₂ (0 to 4,0 mM) or/with 2 mM of EGTA or/with 2 mM of CaCl₂ and various concentrations of Leucettine L41 (L41) (0,1 to 2 μM). Proteins were analyzed by western blot using the α-DYRK1A-Nter antibody. d Control mouse hippocampus extract was incubated at 30 °C during 10 min with 0 mM of CaCl₂ or 2 mM of CaCl₂ or 2 mM of CaCl₂ with L41 at 1 μM. Protein extracts were then immunoprecipitated with the α-DYRK1A-Nter antibody overnight at 4 °C and immunoprecipitated protein extract were analyzed by western blot using α-DYRK1A-Nter, STAT3α and IkBα antibodies