Skip to main content
Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Pathogenic tau modifications occur in axons before the somatodendritic compartment in mossy fiber and Schaffer collateral pathways

Fig. 6

AT8+ tau neurite pathology colocalizes with the axonal marker SMI-312 in the mossy fiber pathway. (a-d). Representative images of AT8 (green), SMI-312 (red) and MAP2 (cyan) triple labeling immunofluorescence staining in the mossy fibers of the hippocampus (merged image in a includes DAPI nuclear counter stain). (a) A low magnification image shows axonal (SMI-312), dendritic (MAP2) and tau+ neurites (AT8) in the CA3 Str. Luc. region of the hippocampus in a Braak stage III case. (b and c) Crossectional analysis of z-stack images (60x magnification, 0.5 μm step size) demonstrate that AT8+ neurites colocalize with SMI-312 (arrows). (d) Cross-sectional analysis of z-stack images show that some AT8+ neurites do not colocalize with SMI-312 or MAP2. Notably, little to no colocalization was observed between AT8+ neurites and MAP2. Scale bars are 20 μm for a and 5 μm for b-d. TNT2+ tau neurite pathology showed similar colocalization with SMI-312, not MAP2 in the mossy fiber pathway (see Additional file 6: Figure S6)

Back to article page

Acta Neuropathologica Communications

ISSN: 2051-5960

Contact us