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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Transcriptomopathies of pre- and post-symptomatic frontotemporal dementia-like mice with TDP-43 depletion in forebrain neurons

Fig. 4

Dendritic alternations of neurons in the cortex of aged TDP-43 cKO mice. Golgi staining was used to visualize the neuronal dendrites in the cortex of 3- and 12-month-old TDP-43 cKO mice in comparison to the Ctrl mice. a Representative images of the Golgi-staining patterns in the left 2 panels show the obvious morphology changes and dendritic shortening of the cortical neuron of 12-month-old TDP-43 cKO mice in comparison to the Ctrl mice. Scale bar is 500 μm. Quantitative comparison of the dendritic lengths of cortical layer V neurons of TDP-43 cKO and Ctrl mice is shown in the right two diagrams. Note the significant reduction of the average dendritic length in 12-month-old TDP-43 cKO. Statistical analysis was done by unpaired t test with the error bars being SEM. P < 0.05 was considered significant. b Enlarged views of cortical layer V neurons. Representative images show the cortical dendrites with segmental beading dendrites (arrow heads) and degenerated spines (arrows), respectively. Scale bar is 20 μm. c Quantifications of the % of cortical layer V neurons with beading dendrites (left histogram) or shorter dendrites (right histogram). Statistically analyzed by unpaired t test with error bars reported as SEM, P < 0.05 was considered significant. Note the markedly increase of abnormal dendrites in TDP-43 cKO mice at the age of 12 months, but not 3 months. d Immunofluorescence staining with anti-neurofilament H (SMI-32) (green) showing a marked decrease of the neuron numbers in the cortical layer III/ V of the retrosplenial cortex (RS) of TDP-43 cKO mouse brain but not Ctrls. Enlarged views of RS region are shown in the upper left corner of each image

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