Fig. 3
From: Heterogeneous nuclear ribonucleoproteins R and Q accumulate in pathological inclusions in FTLD-FUS
hnRNP R and Q are insoluble in FTLD-FUS. Representative immunoblots demonstrating hnRNP R and hnRNP Q in fractions of varying solubility in FTLD-FUS and normal control brains. Proteins were sequentially extracted into high salt (lane 1), RIPA/ 2% SDS (lane 2), and 8 M urea / 8% SDS (lane 3) fractions. 20 μg of protein was loaded from high salt and RIPA-SDS fractions, while 5 μg of protein was loaded from the urea fractions. Two prominent bands were observed for hnRNP R, whilst a single band was observed for hnRNP Q. Only the FTLD-FUS cases showed bands in the insoluble urea fraction for both proteins