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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Emergence of synaptic and cognitive impairment in a mature-onset APP mouse model of Alzheimer’s disease

Fig. 3

Induction of APP expression and increase in Aβ following dox removal in mature mice. a Experimental timeline showing time points at which APP expression and Aβ levels were measured. Hippocampal lysates were obtained from 12 week-old mice expressing the APPSw,Ind transgene from postnatal development (developmental onset) and from mature-onset mice reared on dox to suppress APPSw,Ind expression throughout life (0 days) and in mice 3 days, 2 weeks, 3 weeks and 12 weeks after dox removal. b Representative Western blots probing for APP expression (6E10) in WT, APP, tTA and APP/tTA mice at the different time points tested. GAPDH was used as a loading control. c Normalised APP signal intensity showed a significant effect of time of dox removal (control genotypes n = 9 for all time points, APP/tTA genotype n = 3 for all time points). d A rapid increase in Aβ40 was observed in APP/tTA mice following dox removal (control n = 9, APP/tTA n = 3 for all time points except 12 weeks where APP/tTA n = 7). e A rapid increase in Aβ42 was observed in APP/tTA mice following dox removal (control n = 9, APP/tTA n = 3 for all time points except 12 weeks where APP/tTA n = 7)

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