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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: EphB2-dependent signaling promotes neuronal excitotoxicity and inflammation in the acute phase of ischemic stroke

Fig. 4

EphB2 deficiency mitigates brain inflammation in the acute stage after ischemic stroke. a-c WT and Ephb2−/− mice underwent 60 min MCAO followed by either 6, 12, 24, 48 or 72 h of reperfusion or were subjected to sham surgery. Immunofluorescent staining of Ly6G, Iba-1 and GFAP was applied to determine the number of (a) infiltrating neutrophils within the contra- and ipsilateral brain hemisphere (mean ± SD; n = 4/4; Two-way ANOVA with Holm-Sidak’s multiple comparisons test), (b) microglia/macrophages and (c) astrocytes along the infarct border zone (mean ± SD; n = 4/4; Two-way ANOVA with Holm-Sidak’s multiple comparisons test). d RNA was extracted from ipsilesional brain tissue and corresponding tissue of sham operated mice. Expression of pro-inflammatory factors in brain tissue 12 h upon restoration of MCA perfusion was evaluated by quantitative real-time RT-PCR (mean ± SD; n = 3/3; Two-way ANOVA with Holm-Sidak’s multiple comparisons test). S, sham; M, MCAO. * p < 0.05

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