Table 1 Primary antibodies and modes of antigen retrieval for immunohistochemistry
Antibody | Origin | Dilution | Antigen retrieval | CSA | Source |
|---|---|---|---|---|---|
Amyloid precursor protein (APP) [clone 22C11] | Mouse (mAb) | 1:1000 | St (C) | Chemicon, Merck Millipore; MAB348 | |
CD3 [clone SP7] | Rabbit (mAb) | 1:2000 | St (E) | yes | Neomarkers; RM-9107 |
CD68 [clone ED1] | Mouse | 1:10,000 | St (E) | Serotec | |
2′,3’-Cyclic-nucleotide 3′-phosphodiesterase (CNPase) [clone SMI 91] | Rabbit (mAb) | 1:2000 | St (E) | Sternberger Monoclonals; SMI91 | |
Glial fibrillary acidic protein (GFAP) | Rabbit (pAb) | 1:3000 | St (E) | Dako; Z0334 | |
Ionized calcium-binding adapter molecule 1 (Iba-1) | Rabbit (pAb) | 1:3000 | St (E) | Wako Chemicals; 019–19741 | |
Inducible nitric oxide synthase (iNOS) | Rabbit (pAb) | 1:750 | St (C) | Chemicon, Merck Millipore; AB16311 | |
MHC class II RT1B [clone OX-6] | Mouse (mAb) | 1:250 | St (E) | Serotec; MCA46G | |
Neuronal nuclei (NeuN) [clone A60] | Mouse (mAb) | 1:250 | St (C) | Chemicon, Merck Millipore; MAB377 | |
Chondroitin sulfate proteoglycan 4 (NG2) | Rabbit (pAb) | 1:250 | St (E) | Chemicon, Merck Millipore; AB5320 | |
Oligodendrocyte transcription factor 2 (Olig2) | Rabbit (pAb) | 1:1000 | St (E) | Chemicon, Merck Millipore; AB9610 | |
p22phox [clone FL-195] | Rabbit (pAb) | 1:100 for pre-absorptiona | St (C) | Santa Cruz Biotechnology; sc20781 | |
Purinergic receptor P2Y12 (P2RY12) | Rabbit (pAb) | 1:1000 | St (E) | yes | Kindly provided by Oleg Butovsky (Harvard Medical School) |
Transmembrane protein 119 (TMEM119) | Rabbit (pAb) | 1:1000 | St (E) | Synaptic Systems; 400002 | |
ATP synthase alpha [clone 7H10] | Mouse (mAb) | 1:200 | St (E) | Thermo Fisher Scientific; 459240 | |
Phosphorylated neurofilament H [clone SMI 31] | Mouse (mAb) | 1:20,000 | St (E) | Affiniti; NA1219 |