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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Microglia pre-activation and neurodegeneration precipitate neuroinflammation without exacerbating tissue injury in experimental autoimmune encephalomyelitis

Fig. 3

EAE-induced T cell infiltration differs between Lewis and LEWzizi rats. a, c Quantification of CD3+ T cell-containing perivascular cuffs in the lumbar spinal cord (a) and mesencephalon (c) of 4-month-old (4 M) Lewis and LEWzizi rats at the peak of EAE (6 days post transfer of MBP-specific T cells). b Area fraction analysis of CD3-stained lumbar spinal cord cross sections of 4 M Lewis and LEWzizi rats at the peak of EAE. The percentages of positively labelled area are depicted. d Quantification of parenchymal CD3+ T cells within the mesencephalon of 4 M Lewis and LEWzizi rats at the peak of EAE. e Evaluation of CD3+ inflammatory cuffs in the lumbar spinal cord, medulla oblongata, thalamus/hypothalamus (Thala/Hypothala), cerebellum and mesencephalon (MesEnc) of 4 M Lewis and LEWzizi rats at the peak of EAE. Experimental groups each comprise 6–8 rats. f CD3 immunohistochemical staining of lumbar spinal cord cross sections of 4 M Lewis and LEWzizi rats at the peak of EAE. Scale bars, 500 μm. a-d Graphs represent mean ± SD. Experimental groups each comprise 6–8 rats. ****, p-value < 0.0001; ns, not significant (a, c) Reported statistics result from unpaired two-tailed Student’s t-tests (data pooled according to rat genotype and independent of T cell genotype). b, d Red lines indicate the mean ± SD of age-matched naïve Lewis or LEWzizi controls. Statistics result from two-way ANOVAs (separate analyses for day 6 and day 10) reporting (i) differences between Lewis EAE rats and LEWzizi EAE rats by black bars and (ii) differences between naïve controls rats and EAE rats by orange bars. Data were pooled according to rat genotype and independent of T cell genotype

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