Fig. 5

Expression of SGmic and SGmac genes in glioma-associated microglia and monocytes/macrophages isolated from two different experimental mouse glioma models. (a) RCAS tumors were generated by injection of RCAS-PDFGB into Ntv-a;Ink4a-Arf−/−;Gli-luc mice, and after 5 weeks, glioma-associated microglia were isolated as CD11b⁺, CD45^low, F11r⁺, Ly6G^neg, Sell^neg, CD3^neg, CD19^neg, NK1.1^neg cells, while glioma-associated monocytes/macrophages were isolated as CD11b⁺, CD45^high, F11r⁺, Ly6G^neg, Sell^neg, CD3^neg, CD19^neg, NK1.1^neg cells by FACS. Graph shows RNA sequencing results of the two populations as log₂-fold change expression of glioma-associated microglia to monocytes/macrophages for SGmic (P2ry13, P2ry12, Gpr34, Slc2a5, Siglec-H, Olfml3, Tmem119, Fcrls) and SGmac (F10, Emilin, F5, C3, Gda, Mki67, Sell and Hp) genes, as well as the reference genes (Cx3cr1, CD11b, CD45, Ccr2). Genes not detected in the previous proteomic analysis (see Fig. 4; SGmic: P2ry13, Gpr34 and SGmac: F10, F5) are colored with gradients. Graphs show log₂-fold change expression of the SGmic and SGmac genes in glioma-associated microglia versus monocytes/macrophages isolated from (b) RCAS and (c) GL261 tumors derived from published sequencing data [5]. Glioma-associated microglia were isolated from RCAS tumors based on CD45⁺CD11b⁺Ly6G⁻Ly6C⁻ TdTomato⁺ GFP⁻ expression, glioma-associated monocytes/macrophages were isolated as CD45⁺CD11b⁺Ly6G⁻Ly6C⁻ TdTomato⁻ GFP⁺ cells. GL261 glioma-associated microglia were isolated as CD45⁺CD11b⁺Ly6G⁻Ly6C⁻TdTomato⁺ cells and glioma-associated monocytes/macrophages were isolated as CD45⁺CD11b⁺Ly6G⁻Ly6C⁻ TdTomato⁻ cells