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Table 1 Antibodies employed in this study

From: SET SUMOylation promotes its cytoplasmic retention and induces tau pathology and cognitive impairments

Antibody

Specificity

Type

Dilution

Source

Flag

Detecting Flag fusion sequence

mAb

1:1000 for WB

1:200 for IP

Sigma

SET

Detecting I2PP2A of mouse, rat and human origin

mAb

1:1000 for WB

1:50 for IP

Santa Cruze

SUMO-1

Detecting SUMO-1 and endogenous levels of SUMOylated proteins

mAb

1:1000 for WB

1:50 for IP

Cell Signaling

UBC9

Detecting endogenous levels of total UBC9 protein

mAb

1:1000 for WB

Cell Signaling

PP2A

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

mAb

1:1000 for WB

Millipore

Tau-5

Total-tau

mAb

1:1000 for WB

Lab Vision

pS199

Phosphorylated Tau at Ser199

pAb

1:1000 for WB

Invitrogen

pS396

Phosphorylated Tau at Ser396

pAb

1:1000 for WB

Invitrogen

pS404

Phosphorylated Tau at Ser404

pAb

1:1000 for WB

SAB

Synapsin-1

Detecting Synapsin-1 protein

mAb

1:1000 for WB

Millipore

Synaptophysin

Recognizing synaptophysin in mice brain extracts

mAb

1:1000 for WB

Sigma

Synaptotagmin

Recognizing synaptotagmin in mice brain extract

mAb

1:1000 for WB

Abcam

PSD95

Detecting endogenous levels of total PSD95 protein

mAb

1:1000 for WB

Cell Signaling

PSD93

Detecting endogenous levels of total PSD93 protein

mAb

1:1000 for WB

Cell Signaling

NR2A

N-methyl D-asparate receptor subtype 2A

mAb

1:1000 for WB

Millipore

GluR1

Detecting the Glutamate receptor 1 protein

mAb

1:1000 for WB

Millipore

DM1A

Recognizing alpha-tubulin

mAb

1:1000 for WB

Sigma

  1. mAb Monoclonal antibody, pAb Polyclonal antibody, WB Western blot, IP Immunoprecipitation, IF Immunofluorescence, PP2A Protein phosphatase-2A