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Table 1 Antibodies employed in this study

From: SET SUMOylation promotes its cytoplasmic retention and induces tau pathology and cognitive impairments

Antibody Specificity Type Dilution Source
Flag Detecting Flag fusion sequence mAb 1:1000 for WB 1:200 for IP Sigma
SET Detecting I2PP2A of mouse, rat and human origin mAb 1:1000 for WB 1:50 for IP Santa Cruze
SUMO-1 Detecting SUMO-1 and endogenous levels of SUMOylated proteins mAb 1:1000 for WB 1:50 for IP Cell Signaling
UBC9 Detecting endogenous levels of total UBC9 protein mAb 1:1000 for WB Cell Signaling
PP2A Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform mAb 1:1000 for WB Millipore
Tau-5 Total-tau mAb 1:1000 for WB Lab Vision
pS199 Phosphorylated Tau at Ser199 pAb 1:1000 for WB Invitrogen
pS396 Phosphorylated Tau at Ser396 pAb 1:1000 for WB Invitrogen
pS404 Phosphorylated Tau at Ser404 pAb 1:1000 for WB SAB
Synapsin-1 Detecting Synapsin-1 protein mAb 1:1000 for WB Millipore
Synaptophysin Recognizing synaptophysin in mice brain extracts mAb 1:1000 for WB Sigma
Synaptotagmin Recognizing synaptotagmin in mice brain extract mAb 1:1000 for WB Abcam
PSD95 Detecting endogenous levels of total PSD95 protein mAb 1:1000 for WB Cell Signaling
PSD93 Detecting endogenous levels of total PSD93 protein mAb 1:1000 for WB Cell Signaling
NR2A N-methyl D-asparate receptor subtype 2A mAb 1:1000 for WB Millipore
GluR1 Detecting the Glutamate receptor 1 protein mAb 1:1000 for WB Millipore
DM1A Recognizing alpha-tubulin mAb 1:1000 for WB Sigma
  1. mAb Monoclonal antibody, pAb Polyclonal antibody, WB Western blot, IP Immunoprecipitation, IF Immunofluorescence, PP2A Protein phosphatase-2A