Fig. 9

Aβ oligomers stimulation upregulates SET SUMOylation. a and b Rat primary hippocampal neurons were treated with the indicated concentrations of Aβ oligomers for 24 h. Samples were lysed with RIPA buffer and probed with anti-SET and anti-SUMO-1 antibodies via western blotting analysis. Arrows indicate 52 kDa bands that cross react with both anti-SET (39 kDa) and anti-SUMO-1 (13 kDa) representing endogenously SUMOylated SET induced by Aβ exposure. c and d Quantification of the blots in (a and b). e Rat primary hippocampal neurons were treated with the indicated concentrations of Aβ oligomers . Cells were lysed and immunoprecipitations performed using anti-SUMO-1 antibodies. Pull-downs were subjected to western blotting analysis and probed with anti-SET antibodies. f Quantification of the blots in (e).**P < 0.01, ***P < 0.001 vs. DMSO (0 nM Aβ). All data represent the mean ± SD of 3 independent experiments