Fig. 7

SET SUMOylation leads to PP2A inhibition and tau hyperphosphorylation. a Levels of PP2A in hippocampus tissue homogenates was assessed via western blotting analysis and b quantified using ImageJ. c PP2A activity in hippocampal tissue homogenates was measured using commercial PP2A assays. d Tau phosphorylation in hippocampal tissue homogenates was measured via western blotting analysis with the indicated panel of phosphorylation site-specific antibodies. Blots were normalized to total tau protein probed using Tau-5. Tubulin was used as a loading control. e Tau phosphorylation at Ser396, Ser404, AT8 (Ser202/205) was quantified using ImageJ. f Total tau developed with Tau5 were comparable among each group. All data shown represent the mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.01