Fig. 2

K68 is a major SET SUMOylation site. a SUMOsp2.0 prediction of candidate SET SUMOylation sites. b HEK-293 T cells were transfected with SET-WT, SET-K14R, SET-K36R, SET-K39R or SET-K68R. Cells were lysed and subjected to western blotting analysis with anti-Flag antibodies. c Quantification of the blots in B. ***P < 0.001 vs. WT. All data represent the mean ± SD of 3 independent experiments. d HEK-293 T cells were co-transfected with Flag-SET-WT, Flag-SET-K68R, His-SUMO-1 and UBC9 plasmids for 48 h. Cells were lysed and co-immunoprecipitations performed to detect SET SUMOylation. e HEK-293 T cells were co-transfected with Flag-SET-WT, Flag-SET-K68R and UBC9 for 48 h. Cells were lysed and immunoprecipitations performed using anti-SUMO-1 antibodies. Pull-downs were subjected to western blotting analysis and probed with anti-SET antibodies