Fig. 1

SET is SUMOylated in vitro and in vivo. a HEK-293 T cells were transfected with Flag-SET, His-SUMO-1 and UBC9 for 48 h. Cells were lysed and immunoprecipitations performed using anti-SUMO-1 antibodies. Pull-downs were subjected to western blotting analysis and probed with anti-Flag antibodies. b-d Hippocampal homogenates from 3 × Tg mice/h-tau mice (from the Jackson Laboratory, 3rd generation) /APP/PS1 mice (from the Model Animal Research Center of Nanjing University. 2nd generation) were harvested and immunoprecipitations performed using anti-SUMO-1 antibodies. Pull-downs were subjected to western blotting analysis and probed with anti-SET antibodies