Fig. 1

Schematic representation of the in vitro model of CAA used in this study. Sepharose 4 Fast Flow beads are highly cross-linked 4% agarose beads with a mean diameter of 90 μm. The pore sizes of the beads may be similar to the sizes of BM components, resulting in the conjugation of BM components onto the surface of beads. During the rotation, beads are exposed to the relative countercurrent of the reaction mixture, which mimics intramural periarterial drainage (IPAD) flow in vitro and enhances the interaction of Aβ with surface-bound BM components leading to Aβ aggregation. HSA: human serum albumin (modified from Fig. 6 of [10])