Skip to main content

Advertisement

Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Enhanced phosphorylation of T153 in soluble tau is a defining biochemical feature of the A152T tau risk variant

Fig. 5

Accumulation of soluble pT153 is specific to A152T carriers. a Recombinant wild-type (WT) and mutant tau proteins (including A152T, A152T/T153A, and T153A) were phosphorylated in vitro with GSK3β, and subsequently evaluated by immunoblotting. 100 ng of each recombinant phosphorylated protein was loaded, followed by detection with phospho-specific antibody (pT153) and E1 (human-tau specific antibody) to confirm equal protein loading. b Biochemical fractionation followed by immunoblotting reveals significant accumulation of pT153 in the S1 and S2 soluble fractions in A152T-AAV mice, while pT153-positive tau is detected in the P3 fraction in P301L-AAV mice. c The amount of pT153 and total tau in the soluble S1 fraction from frontal cortex of A152T carriers and noncarriers with Alzheimer’s disease (AD) was evaluated by immunoblotting

Back to article page