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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Absence of endothelial α5β1 integrin triggers early onset of experimental autoimmune encephalomyelitis due to reduced vascular remodeling and compromised vascular integrity

Fig. 3

Accelerated leukocyte infiltration and demyelination in α5-EC-KO mice during EAE progression. a and d. Frozen sections of lumbar spinal cord taken from α5-EC-KO and WT littermate control mice at the pre-symptomatic and peak symptomatic phases of EAE were stained using antibodies specific for the inflammatory leukocyte marker CD45 (AlexaFluor-488) and fluoromyelin-red (FM) in panel a and for the leukocyte marker Mac-1 (AlexaFluor-488) in panel d. Quantification of CD45 (b), extent of demyelination (c) or Mac-1 (e) fluorescent signal at different time-points of EAE progression. Results are expressed as the mean ± SEM (n = 4 mice/group). Note that in the pre-symptomatic phase of EAE, α5-EC-KO mice show elevated levels of both inflammatory leukocyte markers CD45 and Mac-1 and increased levels of demyelination, as measured by a reduced fluoromyelin signal. * p < 0.05 vs. WT

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