Table 2 Primary antibodies used in this study
From: Amyloid-beta and phosphorylated tau in post-mortem Alzheimer’s disease retinas
Group | Name | Epitope | Source | Concentration |
|---|---|---|---|---|
APP/Aβ | 12F4 | Amyloid-beta1–42 | Biolegend San Diego, CA, USA | 1:100 |
6E10 | Amyloid-beta1–16 | Covance, BioLegend, San Diego, CA, USA | 1:800 | |
4G8 | Amyloid-beta17–24 | Biolegend, San Diego, CA | 1:8000 | |
APP | APP Y188, c-terminal (aa 750) | ABCAM, Cambridge, UK | 1:6000 | |
Tau | HT7 | Tau between residue 159 and 163 | Invitrogen, Thermo Fisher Scientific, MA, USA | 1:8000 |
AT-270 | Tau phosphorylated at Thr 181 | Thermo Scientific, MA, USA | 1:100 | |
AT8 | Tau phosphorylated at Ser 202, Thr205 | Innogenetics, Ghent, Belgium | 1:800 | |
AT-100 | Tau phosphorylated Ser 212, Thr 214 | Thermo Scientific, MA, USA | 1:1000 | |
Anti-pS422 | Tau Fibrils | ABCAM, Cambridge, UK | 1:400 | |
MC-1 | Paired helical filaments | Peter Davies, Pathology and Neuroscience, Donald and Barbara Zucker School of Medicine, Northwell, NY, USA [21] | 1:1000 | |
Other | Curcumin | Fibrillar Aβ and CAA | Sigma Aldrich, St. Louis, MO, USA | 0,1 mg/ml in NaOH |
Thioflavin | Amyloid | Sigma Aldrich, St. Louis, MO, USA | 1% in distilled water | |
Kluver-PAS | Polysaccharides | Kluver: Luxol fast blue, BDH laboratory supplies, Avantor, PA, USA, PAS: Schiff’s reagens, VWR, Avantor, PA, USA | 0.1% in alcohol 96% n.a. |