Skip to main content

Table 2 Primary antibodies used in this study

From: Amyloid-beta and phosphorylated tau in post-mortem Alzheimer’s disease retinas

Group Name Epitope Source Concentration
APP/Aβ 12F4 Amyloid-beta1–42 Biolegend San Diego, CA, USA 1:100
6E10 Amyloid-beta1–16 Covance, BioLegend, San Diego, CA, USA 1:800
4G8 Amyloid-beta17–24 Biolegend, San Diego, CA 1:8000
APP APP Y188, c-terminal (aa 750) ABCAM, Cambridge, UK 1:6000
Tau HT7 Tau between residue 159 and 163 Invitrogen, Thermo Fisher Scientific, MA, USA 1:8000
AT-270 Tau phosphorylated at Thr 181 Thermo Scientific, MA, USA 1:100
AT8 Tau phosphorylated at Ser 202, Thr205 Innogenetics, Ghent, Belgium 1:800
AT-100 Tau phosphorylated Ser 212, Thr 214 Thermo Scientific, MA, USA 1:1000
Anti-pS422 Tau Fibrils ABCAM, Cambridge, UK 1:400
MC-1 Paired helical filaments Peter Davies, Pathology and Neuroscience, Donald and Barbara Zucker School of Medicine, Northwell, NY, USA [21] 1:1000
Other Curcumin Fibrillar Aβ and CAA Sigma Aldrich, St. Louis, MO, USA 0,1 mg/ml in NaOH
Thioflavin Amyloid Sigma Aldrich, St. Louis, MO, USA 1% in distilled water
Kluver-PAS Polysaccharides Kluver: Luxol fast blue, BDH laboratory supplies, Avantor, PA, USA, PAS: Schiff’s reagens, VWR, Avantor, PA, USA 0.1% in alcohol 96% n.a.