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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Low-level blast exposure disrupts gliovascular and neurovascular connections and induces a chronic vascular pathology in rat brain

Fig. 7

Disruption of neurovascular interactions by low-energy blast exposures. Isolated brain vascular fractions were prepared from five control and five blast-exposed rats (6 weeks after blast exposure, same samples as shown in Fig. 5a). a Western blot analysis of the expression of NFH, α-INX and NFM in isolated vascular fractions. The top GAPDH panel represents the loading control for the NFH blot; the bottom GAPDH panel is the loading control for the α-INX and NFM blots. Blots were obtained by sequential probing of the same membrane. All lanes were loaded with 10 μg of protein and contain protein from individual animals. Quantitation is shown in the right panels with expression normalized to GAPDH. Statistical differences were assessed with unpaired t-tests (** p < 0.01, *** p < 0.001, n = 5/group). b Isolated large cerebral vessel, likely a pial or penetrating arteriole, stained for NFH (green) and Griffonia simplicifolia isolectin B4 (red). Nuclei were stained with DAPI (blue). An arrow indicates an NFH-immunostained process that remains attached to the vessel. Other patches of focal NFH immunoreactivity are also visible. Scale bar, 20 μm

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