Fig. 6

Effect of Latrunculin B on transfer of mitochondria and NSC survival. Representative confocal images of Neuronal stem cells (NSCs) stained with cell tracker blue (CTB) and subsequently co-cultured for 17 h with mesenchymal stem cells (MSC) transfected with mito-mcherry (a-d) to label the MSC-derived mitochondria. Neuronal stem cells (NSCs) were treated with 1 μM cisplatin or medium for 8 h followed by co-culture with mesenchymal stem cells (MSCs) treated with 2 μm Latrunculin B (MSC LatB, b and d), control MSC (MSC Ctl, a and c), or without MSC for 17 h. Mitochondrial transfer (e) and NSC survival (f) were quantified as in Figs. 1 and 3. Data are represented as means ± SEM of 4 independent experiments and were analyzed using two-way ANOVA followed by Bonferroni’s post-hoc test. ** P < 0.01; * P < 0.05