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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Co-occurrence of chronic traumatic encephalopathy and prion disease

Fig. 5

Conformational stability and solubility assay (CSSA) of PrPD species. Solubility and stability of totPrPD (PK-) and resPrPD (PK+) were measured as [GdnHCl]½ molar values, which denotes the molar concentration competent to solubilize half of the substrate. a and b: No significant difference related to totPrPD and resPrPD was detected between each of the CTE cases 1–3 and their respective controls (n = 3). By contrast, both totPrPD and resPrPD values were significantly different in sCJDMM1 and sCJDMM2 as expected. Mean [GdnHCl]½ molar values for totPrPD and resPrPD were: in CTE case 1, 1.44 and 1.8, respectively, and 1.58 ± 0.11 and 1.95 ± 0.13 in controls; in case 2, 1.49 and 1.68, with 1.5 ± 0.04 and 1.69 ± 0.14 in controls; in case 3, 1.78 and 1.93, with 1.43 ± 0.08 and 1.84 ± 0.02 in controls. Mean totPrPD and resPrPD values were 1.43 ± 0.08 and 1.84 ± 0.02 and 1.02 ± 0.06 and 1.22 ± 0.06 in sCJDMM1 and sCJDMM2 respectively. * p ≤ 0.05, *** p ≤ 0.001. c and d: Representative immunoblots of total and resPrPD of CTE cases 1–3 and controls probed with Ab 3F4 at increasing concentrations of GdnHCl

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