Fig. 9
From: GJA1 (connexin43) is a key regulator of Alzheimer’s disease pathogenesis
Gja1 deficiency reduces spontaneous neuronal activity in cortical neuron/astrocyte cocultures as determined by multielectrode array. a. Experimental scheme is indicated. One day prior to cortical neuron preparation and plating, wildtype or Gja1−/− astrocytes were plated onto 48-well MEA array plate in quadruplicate wells. Spontaneous activities of the cocultures were recorded at day 10, 14, 18 and 20 on Axion Maestro. At day 16, cocultures were treated with 10 μM Aβ1–42 oligomers. b. Boxplots of the indicated metrics from the MEA recordings are shown. Each circles represent mean values of individual well, and the horizontal bars within the box indicates means of quadruplicates. Mann-Whitney U tests were used for statistical analysis. * p < 0.05