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Fig. 8 | Acta Neuropathologica Communications

Fig. 8

From: GJA1 (connexin43) is a key regulator of Alzheimer’s disease pathogenesis

Fig. 8

Gja1 deficiency causes reduction of Apoe production and promotes Aβ production in neuron/astrocyte cocultures. a. Quantitative PCR analysis of Apoe in wildtype (WT) and Gja1−/− astrocytes. b. Apoe, Cx43, and actin levels in wildtype and Gja1−/− astrocytes treated with the indicated concentration of Aβ1–42 oligomers for 48 h were determined by immunoblot analysis of cell lysates. c. Apoe secreted into the conditioned medium of astrocytes in B was quantitatively determined by specific ELISA in quadruplicate wells. d and e. Amyloidβ secreted to the conditioned medium of wildtype cortical neurons cocultured with either wildtype or Gja1−/− astrocytes was quantitatively determined by Aβ40 and Aβ42 specific ELISA

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