Fig. 6

GJA1 inhibitors downregulate Gja1 and Apoe, and other network genes. a. Wildtype primary mouse astrocytes were treated with 200 μM carbenoxolone (CBX, gap junction inhibitor) or Lanthanum (La3+, hemichannel inhibitor) for 3 days and levels of Cx43, Apoe were analyzed by immunoblot. b. Quantitative analysis of Cx43 and Apoe protein levels were normalized to Actin. ANOVA followed by Bonferroni post-hoc tests are indicated by asterisks. ** p < 0.01, *** p < 0.001. c. Quantitative gene expression analysis in wildtype astrocytes treated similar to A was performed to analyze GJA1 network drivers. Statistical analysis was performed as in b, and only the comparisons to control are shown. Results representative of two independent experiments are shown for all experiments