Fig. 4

Network analysis on GJA1 genetic networks. a. BN (Bayesian Network) based on astrocyte (khiki) module. Gja1−/− DEG signatures were projected onto BN to detect overlapped gene signatures, which were classified into eight categories: up-regulated only in AST (in red upright triangle), down-regulated only in AST (in blue downside triangle), only up-regulated in AST + NEU (red square), only down-regulated in AST + NEU (blue square), up-regulated in both AST and AST + NEU (in pink diamond), up-regulated in AST but down-regulated in AST + NEU (in pink hexagon), down-regulated in both AST and AST + NEU (in gray diamond), and down-regulated in AST but up-regulated in AST + NEU (in gray hexagon)). In enlarged label were genes that were validated experimentally. Gja1 was in black. In turquoise circle were genes non-overlapped. AST, Gja1−/− astrocyte culture while AST + NEU, Gja1−/− astrocyte culture in the presence of co-cultured neurons. b. GJA1 centered genetic networks inferred by projecting Gja1−/− DEG signatures onto Gja1 correlation concensus network (CGCCS(6)). Overlapped gene nodes were denoted as in A