Fig. 10

Gja1 deficiency reduces stimulated neuronal activity in cortical neuron/astrocyte cocultures as determined by multielectrode array. a and b. Cocultures were stimulated by 3 cycles of voltage-controlled biphasic stimulus from all electrodes within the wells at 14 days (a) and 20 days (b) in vitro. At least 5 times of repeated stimulations were recorded and analyzed. c. Two representative raster plots as recorded on day 20 are shown for wildtype neurons cocultured with wildtype astrocytes (upper panel) or with Gja1−/− astrocytes (lower panel). Y-axis represents 16 electrodes and X-axis represents time after stimulation indicated by red arrowhead. Black horizontal scale bar indicates 100 milliseconds (ms). Each vertical bar represents a detected spike