Fig. 1

Overview of the integrative network analyses and validation experiments performed in the study. a. GJA1 mRNA expression changes and its correlations with clinical and pathological traits were systematically investigated in 29 datasets. GJA1-centric coexpression and regulatory networks. b. Workflow of in vitro functional validation study. Wildtype and Gja1−/− astrocytes with or without wildtype neurons were used to prepare RNA for sequencing and to perform various functional validations (c). d. GJA1’s gene signatures between wildtype and Gja1−/− astrocytes and between coculture of wildtype astrocytes and wildtype neurons and coculture of Gja1−/− astrocytes and wildtype neurons were identified from RNA-sequencing data from the experiments in b. e. GJA1’s gene signatures were used to validate the network structures predicted from the transcriptomic datasets in human AD brains. Functional relevance of GJA1’s gene signatures was also investigated