Fig. 4
From: Identification of TCERG1 as a new genetic modulator of TDP-43 production in Drosophila
CG42724-mediated regulation of TDP-43 production depends mainly on the presence of the TDPBR region. Western Blot analyses of proteins extracted from transgenic flies expressing the UAS-TDP-43_TDPBR or the UAS-TDP-43 constructs, in the presence or the absence of the P(UY)5237 element, under the control of the GMR-Gal4 driver line. Control flies: GMR-Gal4 > +. a Blots were probed with an anti-TDP-43 antibody and representative blots are presented (n ≥ 7). Total protein was used as the loading control. b The normalized expression of the TDP-43 protein is reported in the graphs (mean ± SEM). Genotypes GMR-Gal4 > UAS-TDP-43_TDPBR and GMR-Gal4 > UAS-TDP-43 were arbitrarily set at 100 arbitrary units. Protein levels were compared between both genotypes by using Student’s t-test. ***: p < 0.001. The P(UY)5237 element caused a drastic increase of TDP-43 protein steady-state levels in the context of the UAS-TDP-43_TDPBR construct (n = 8, p = 0.0001), but only a slight rise when the UAS-TDP-43 construct was expressed (n = 7, p = 0.0002)