Fig. 3
From: Identification of TCERG1 as a new genetic modulator of TDP-43 production in Drosophila
CG42724-mediated increase of TDP-43 production results in the appearance of insoluble TDP-43 aggregates and causes cellular toxicity in Drosophila retina. a Light micrographs of newborn Drosophila adult eyes raised at 23 °C. Compared to control flies (GMR-Gal4x2 > +), TDP-43_TDPBRx2 (GMR-Gal4x2 > UAS-TDP-43_TDPBRx2) expression alone triggered no structural defects. Flies overexpressing CG42724 (GMR-Gal4 x2 > UY5237) displayed alteration of the external eye aspect (“rough-eye phenotype”). Coexpression of CG42724 and TDP-43_TDPBRx2 (GMR-Gal4 x2 > UAS-TDP-43_TDPBRx2, UY5237) enhanced the severity of the “rough-eye phenotype” in a synergistic manner. b Western blot analyses of TDP-43 proteins extracted from flies expressing TDP-43_TDPBRx2 with or without the P(UY)5237 transposon under the control of the GMR-Gal4 driver, and control flies bearing only the GMR-Gal4 transgene. Proteins were sequentially extracted in RIPA (soluble) and Urea (insoluble) buffers. Samples were loaded with (+ DTT) or without (− DTT) reducing agent. Blots were probed with an anti-TDP-43 antibody and representative blots are presented (n = 4). Total protein was used as the loading control. CG42724-mediated increased expression of TDP-43 resulted in appearance of DTT-sensitive high molecular weight (HMW) species