Fig. 2
From: Identification of TCERG1 as a new genetic modulator of TDP-43 production in Drosophila
Characterization of the transposon UY5237 line. a The CG42724 transcription unit is represented by the filled rectangle. Exons are represented by rectangles below the transcription unit, and introns as a line. The arrow represents the orientation of transcription from the P{y +}UAS transposon in the UY5237 transgenic line. Scale bar (upper right) is 1000 bp. Schematic representation adapted from FlyBase. (b, c) Quantification of the CG42724 mRNA steady-state levels by RT-QMPSF experiments. Total RNA were extracted from GMR-Gal4 > + (control), GMR-Gal4 > UY5237, GMR-Gal4 > UAS-CG42724RNAi #33737 or GMR-Gal4 > UAS-CG42724RNAi #55357 transgenic flies. The graph represents mean ± SEM after normalization with Cyp1 (reference gene). Controls were arbitrarily set at 100 arbitrary units. The mRNA levels were compared between both genotypes by using Student’s t-test. **: p < 0.01. b CG42724 mRNA expression in flies heterozygous for the UY5237 transposon was significantly increased, compared to control flies (n = 8, p = 0.009). c Expression of RNAi constructs targeting CG42724 (#33737 or #55357) significantly reduced CG42724 mRNA steady-state levels, relative to control flies (CG42724RNAi#33737 n = 4, p = 0.0011, CG42724RNAi#55357 n = 3, p = 0.0037)