Fig. 1
From: Identification of TCERG1 as a new genetic modulator of TDP-43 production in Drosophila
CG42724 expression modulates TDP-43 production. a-c Western Blot analyses of proteins extracted from transgenic flies expressing UAS-TDP-43_TDPBR (a), UAS-FUS (b) or UAS-LacZ (c) constructs, in the presence or the absence of the P(UY)5237 element, under the control of the GMR-Gal4 driver line. Control flies: GMR-Gal4 > +. Expression of TDP-43, FUS and LacZ proteins was qualified using specific antibodies. Representative blots are shown (n ≥ 4). Total protein was used as loading control and the normalized expression of the proteins of interest is reported in the graphs (mean ± SEM). Genotypes GMR-Gal4 > UAS-TDP-43_TDPBR, GMR-Gal4 > UAS-FUS and GMR-Gal4 > UAS-LacZ were arbitrarily set at 100 arbitrary units. The P(UY)5237 element caused a drastic increase of TDP-43 protein steady-state levels (n = 8, p = 0.0001), but did not modify significantly FUS (n = 4, p = 0.524) or LacZ (n = 4, p = 0.822) expression. d, e Western Blot analyses of total proteins extracted from transgenic flies expressing UAS-TDP-43_TDPBR with or without RNAi constructs targeting CG42724 (#33737 or #55357) under the control of the GMR-Gal4 driver line (d). Blots were probed with an anti-TDP-43 antibody and representative blots are presented (n = 4). Total protein was used as the loading control. The normalized expression of the TDP-43 protein is reported in the graphs (mean ± SEM) (e). Control (GMR-Gal4 > UAS-TDP-43_TDPBR) was arbitrarily set at 100 arbitrary units. The reduction of CG42724 expression decreased TDP-43 production by about 50% (CG42724RNAi#33737: n = 4, p = 0.0659, CG42724RNAi#55357: n = 4, p = 0.0040). a-e Protein levels were compared between both genotypes by using Student’s t-test. ns: not significant, **: p < 0.01. ***: p < 0.001