Fig. 7
Age-associated changes in whole-brain transcription were unaffected by microglial repopulation. a Adult (6–8 weeks old) and aged (16–18 months old) male BALB/c mice were provided diets formulated with vehicle or CSF1R antagonist (PLX5622) for 21 d. After 21 d, all mice were provided vehicle diet for an additional 21 d to allow for repopulation of microglia (Repop). After 21 d of repopulation, a 1-mm coronal brain section was isolated from each brain and whole-tissue RNA was extracted and sequenced. b Heat map shows mean expression of 207 genes significantly increased by age (Aged Control vs. Adult Control: Padj < 0.05). These are divided into categories based on patterns of expression following microglial repopulation: Exacerbated (Aged Repop vs. Aged Control: P < 0.05; conserved directionality), Partially Reversed (Aged Repop vs. Aged Control: P < 0.05; Aged Repop vs. Adult Control: P < 0.05), or Reversed (Aged Repop vs. Aged Control: P < 0.05; Aged Repop vs. Adult Control: P ≥ 0.05). c Heat map showing mean expression of 202 genes significantly decreased by age subdivided as described above. Heat maps are normalized by row and selected genes are shown for each category (n = 6 mice / group)