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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Forced turnover of aged microglia induces an intermediate phenotype but does not rebalance CNS environmental cues driving priming to immune challenge

Fig. 4

Depletion and repopulation of microglia partially reversed the microglial aging transcriptional signature. a Adult (6–8 weeks old) and aged (16–18 months old) male BALB/c mice were provided diets formulated with vehicle or CSF1R antagonist (PLX5622) for 21 d. After 21 d, all mice were provided vehicle diet for an additional 21 d to allow for repopulation of microglia (Repop). After 21 d of repopulation, Percoll-enriched microglia were sorted, and RNA was collected and sequenced. b PCA plot shows unsupervised clustering of treatment groups by age (PC1). c Heat map shows mean expression of 455 genes significantly increased by age (Aged Control vs. Adult Control: Padj < 0.05 and fold change > 1.5). These are divided into categories based on patterns of expression following microglial repopulation: Exacerbated (Aged Repop vs. Aged Control: P < 0.05; conserved directionality), Partially Reversed (Aged Repop vs. Aged Control: P < 0.05; Aged Repop vs. Adult Control: P < 0.05), or Reversed (Aged Repop vs. Aged Control: P < 0.05; Aged Repop vs. Adult Control: P ≥ 0.05). d Heat map showing mean expression of 56 genes significantly decreased by age and subdivided as described above. Heat maps are normalized by row and selected genes are shown for each category (n = 6 mice/group)

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