Fig. 2

Microglia repopulated independent of age after CSF1R antagonist-mediated depletion. a Adult (6–8 weeks old) and aged (16–18 months old) male BALB/c mice were provided diets formulated with vehicle or CSF1R antagonist (PLX5622) for 21 d. After 21 d, all mice were provided vehicle diet for up to 21 d to allow for repopulation of microglia. After 0–21 days of repopulation, the number of microglia was determined in the cortex. b Representative Iba1 immunolabeling in the cortex of adult and aged mice at baseline and after 0, 1, 3, 5, 7, and 21 d of repopulation. Scale bar = 100 μm. c Number of Iba1⁺ microglia in the cortex at baseline and with 0, 1, 3, 5, 7 and 21 days of repopulation (n = 4–7 mice/group). Graphs represent the mean ± SEM. Means with * are different from age-matched controls (P < 0.05)